human multiple myeloma cell line im-9  (Korean Cell Line Bank)

Journal: Cell reports

Article Title: A MYC-controlled redox switch protects B lymphoma cells from EGR1-dependent apoptosis.

doi: 10.1016/j.celrep.2023.112961

Figure Lengend Snippet: Figure 1. N-acetylcysteine and vitamin C selectively kill human B lymphoma cell lines and inhibit tumor growth in vivo (A) Viability of human B lymphoma cell lines following 48 h incubation with N-acetylcysteine (NAC; left) and vitamin C (VitC; right) as judged by PrestoBlue-based assays. (B) Number of cells remaining after incubation with NAC and VitC for 72 h. (C) Number of Ramos (left) and LY1 (right) cells remaining after incubation with lower doses of NAC, VitC, and both compounds for 48 h. (D and E) Tumor growth in NSG mice injected s.c. with 107 Ramos (left) and LY1 (right) cells. When tumors had developed, the mice were injected i.p. with PBS (Ctrl, n = 11) and higher doses of NAC (n = 7) and VitC (n = 7) (D) and lower doses of NAC (n = 13), VitC (n = 13), and both NAC and VitC (n = 7) (E) five times/week; tumors were measured three times/week. (F) Tumor growth in NSG mice engrafted subcutaneously with 107 IM9 cells. When tumors had developed, the mice were injected i.p. with PBS (Ctrl, n = 5), NAC (n = 5), and VitC (n = 5) five times/week. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dextran solution Pharmacia Cat#Dextran T-500 NH4Cl StemCell Cat#07800 FxCycle PI/RNase Staining Solution Invitrogen Cat#1985233 MethoCult medium Stemcell Technologies Cat#03630 Chemicals, peptides, and recombinant proteins N-acetylcysteine Sigma Cat#A7250 Vitamin C Sigma Cat#A5960 L-Glutathione Sigma Cat#G4251 L-Cysteine Sigma Cat#C8755 I-BET762 (SML1272-5MG), Sigma Cat#SML1272-5MG L-Buthionine-sulfoximine Sigma Cat#B2515 Z-VAD-FMK Sigma Cat#V116-2MG Doxycycline Sigma-Aldrich Cat#D1822 Non-Essential Amino Acids ThermoFisher Cat#11140050 PrestoBlue Cell Viability Reagent ThermoFisher Cat#A13262 Polybrene Sigma-Aldrich Cat#107689-10G Urea Sigma-Aldrich Cat#U0631 Critical commercial assays GSH/GSSG-Glo assay Promega Cat#V6611 Alexa Fluor 488 Annexin V ThermoFisher Cat#A13201 Propidium iodide (PI) Biolegend Cat#421301 Complete protease inhibitor cocktail ThermoFisher Cat#78430 Dynabeads Protein G Immunoprecipitation Kit ThermoFisher Cat#10007D ROS-Glo-H2O2 assay Promega Cat#G8820 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 Deposited data Raw and analyzed data This paper GSE199843; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE199843 Experimental models: Cell lines IM9 ATCC Cat#CCL-159 BL2 DSMZ Cat#ACC-625 L428, LY1, LY3, Karpas-422, DB, Daudi, and Ramos Gift from Dr. S. Okret, Karolinska Institutet N/A P493-6, and Jurkat Gift from Dr. Y. Bryceson, Karolinska Institutet N/A TALL1 DSMZ Cat#ACC 521 Experimental models: Organisms/strains lMYC mice C57BL/6–129/Sv mixed genetic background.

Techniques: In Vivo, Incubation, Injection

Journal: Cell reports

Article Title: A MYC-controlled redox switch protects B lymphoma cells from EGR1-dependent apoptosis.

doi: 10.1016/j.celrep.2023.112961

Figure Lengend Snippet: Figure 1. N-acetylcysteine and vitamin C selectively kill human B lymphoma cell lines and inhibit tumor growth in vivo (A) Viability of human B lymphoma cell lines following 48 h incubation with N-acetylcysteine (NAC; left) and vitamin C (VitC; right) as judged by PrestoBlue-based assays. (B) Number of cells remaining after incubation with NAC and VitC for 72 h. (C) Number of Ramos (left) and LY1 (right) cells remaining after incubation with lower doses of NAC, VitC, and both compounds for 48 h. (D and E) Tumor growth in NSG mice injected s.c. with 107 Ramos (left) and LY1 (right) cells. When tumors had developed, the mice were injected i.p. with PBS (Ctrl, n = 11) and higher doses of NAC (n = 7) and VitC (n = 7) (D) and lower doses of NAC (n = 13), VitC (n = 13), and both NAC and VitC (n = 7) (E) five times/week; tumors were measured three times/week. (F) Tumor growth in NSG mice engrafted subcutaneously with 107 IM9 cells. When tumors had developed, the mice were injected i.p. with PBS (Ctrl, n = 5), NAC (n = 5), and VitC (n = 5) five times/week. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dextran solution Pharmacia Cat#Dextran T-500 NH4Cl StemCell Cat#07800 FxCycle PI/RNase Staining Solution Invitrogen Cat#1985233 MethoCult medium Stemcell Technologies Cat#03630 Chemicals, peptides, and recombinant proteins N-acetylcysteine Sigma Cat#A7250 Vitamin C Sigma Cat#A5960 L-Glutathione Sigma Cat#G4251 L-Cysteine Sigma Cat#C8755 I-BET762 (SML1272-5MG), Sigma Cat#SML1272-5MG L-Buthionine-sulfoximine Sigma Cat#B2515 Z-VAD-FMK Sigma Cat#V116-2MG Doxycycline Sigma-Aldrich Cat#D1822 Non-Essential Amino Acids ThermoFisher Cat#11140050 PrestoBlue Cell Viability Reagent ThermoFisher Cat#A13262 Polybrene Sigma-Aldrich Cat#107689-10G Urea Sigma-Aldrich Cat#U0631 Critical commercial assays GSH/GSSG-Glo assay Promega Cat#V6611 Alexa Fluor 488 Annexin V ThermoFisher Cat#A13201 Propidium iodide (PI) Biolegend Cat#421301 Complete protease inhibitor cocktail ThermoFisher Cat#78430 Dynabeads Protein G Immunoprecipitation Kit ThermoFisher Cat#10007D ROS-Glo-H2O2 assay Promega Cat#G8820 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 Deposited data Raw and analyzed data This paper GSE199843; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE199843 Experimental models: Cell lines IM9 ATCC Cat#CCL-159 BL2 DSMZ Cat#ACC-625 L428, LY1, LY3, Karpas-422, DB, Daudi, and Ramos Gift from Dr. S. Okret, Karolinska Institutet N/A P493-6, and Jurkat Gift from Dr. Y. Bryceson, Karolinska Institutet N/A TALL1 DSMZ Cat#ACC 521 Experimental models: Organisms/strains lMYC mice C57BL/6–129/Sv mixed genetic background.

Techniques: In Vivo, Incubation, Injection